目的 建立冬虫夏草与其红花色素染色伪品鉴别研究方法。方法 在传统鉴别经验基础上,应用多种显微鉴别技术,并结合高效液相色谱法(HPLC)、超高效液相色谱质谱联用技术(UPLC-MS/MS),对本次收集的冬虫夏草与其染色伪品进行鉴别研究。结果 在冬虫夏草染色伪品中可观察到红花的花粉粒特征,并可检测到红花专属性成分羟基红花黄色素A。结论 本研究建立的性状、显微特征和化学成分确认的综合分析方法可准确筛查出冬虫夏草中红花色素染色的造假情况,为中药全生命周期之质量管理提供技术手段。
Abstract
OBJECTIVE To establish a method for identification of Dongchongxiacao(Cordyceps sinensis)and its counterfeits dyed by safflower plant pigment. METHODS On the basis of traditional identification experience, a variety of microscopic identification techniques were applied, combined with HPLC and UPLC-MS / MS, Cordyceps sinensis and its dyed counterfeits collected in this study were identified. RESULTS The micrioscopic feature of pollen grain of safflower were observed in the dyed counterfeits, and the specific component of hydroxy safflower yellow A was further detected. CONCLUSION The comprehensive analysis method based on “morphology-tissue-chemical composition” established in this study can accurately screen the counterfeiting of safflower plant pigment staining in Cordyceps sinensis. This method provides technical methods for the quality management of TCM life cycle.
关键词
冬虫夏草 /
植物色素 /
红花 /
鉴别
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Key words
Cordyceps sinensis /
plant pigment /
safflower flower /
identification
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中图分类号：
R282
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参考文献
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脚注
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基金
青海省重大科技专项资助(2021-SF-A4)
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